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Medical Laboratory Reagents

Binuclease
CAS No. 9025-65-4     EC No. 3.1.30.2
from yeast cells with cloned gene encoding genetically engineered Serratia marcescens
Catalog/Part No.    CT-B-021
Synonyms Serratia Marcescens Binuclease; Unrestricted Endonuclease
Properties
pH range pH 6~10
optimum pH 8.0
Storage Temperature -20 C for lyophilized powder
Form lyophilized powder
Temperature profile Optimum temperature 37C
Temperature range of enzyme activity: 0~42C
Specific activity = 1000 kU/mg protein
Unit definition: According to the protocol from Sigma-Aldrich, one unit of Binuclease will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ?A260nm of 1.0 in 30 minutes at pH 8.0 at 37C.
Activity = 20 kU/mg lyophilized powder
= 250 U/L buffered aqueous glycerol solution
Purity and Quality
Protease: None detected
Endotoxin (Tachypleus Amebocyte Lysate): None Detected
Product Description
General Description Binuclease is a genetically engineered endonuclease from Serratia marcescens gene that can digest all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5 monophosphate terminated oligonucleotides 2-5 bases in length. Binuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester.
Advantages Binuclease is produced from eukaryotic yeast cells that avoid the contamination of endotoxin from prokaryotic cells. The specific activity of Binuclease was meatured at = 1000 kU/mg protein, which is much higher than any existing products (as much as 200%). Binuclease is highly stable under harsh industrial conditions and very cost effective, which make it a perfect alternative for Benzonase.
Application: Binuclease is an ideal tool to remove nucleic acids (DNA and RNA) in biological products or reduce viscosity in cell lysates. Endonuclease is widely used in the pharmaceutical industry to eliminate nucleic acid contamination in vaccine and protein products.
Preparation Notes
Activators 1~10 mM Mg2+
Stability lyophilized powder is stable for 3 years when stored below 4C. Buffered aqueous glycerol solution is stable for 1 years when stored at -20C.
Transportation and Storage:
Buffered aqueous glycerol solution: transport with blue ice (below 4C ). Storage Recommended at -20C
lyophilized powder: transport at room temperature. Storage Recommended below 4C.
Storage buffer: 20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 20 mM NaCl, 50% glycerol.
Dilution buffer: 20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 20 mM NaCl.
Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses.



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